Affiliation:
1. Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA
2. Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA
3. Department of Global Health, Pathobiology Interdisciplinary Program, University of Washington, Seattle, Washington, USA
Abstract
ABSTRACT
Mycoplasma genitalium
is an underappreciated cause of human reproductive tract disease, characterized by persistent, often asymptomatic, infection. Building on our previous experiments using a single female pig-tailed macaque as a model for
M. genitalium
infection (G. E. Wood, S. L. Iverson-Cabral, D. L. Patton, P. K. Cummings, Y. T. Cosgrove Sweeney, and P. A. Totten, Infect Immun 81:2938–2951, 2013,
https://doi.org/10.1128/IAI.01322-12
), we cervically inoculated eight additional animals, two of which were simultaneously inoculated in salpingeal tissue autotransplanted into abdominal pockets. Viable
M. genitalium
persisted in the lower genital tract for 8 weeks in three animals, 4 weeks in two, and 1 week in one; two primates resisted infection. In both animals inoculated in salpingeal pockets, viable
M. genitalium
was recovered for 2 weeks. Recovery of viable
M. genitalium
from lower genital tract specimens was improved by diluting the specimen in broth and by Vero cell coculture. Ascension to upper reproductive tract tissues was not detected, even among three persistently infected animals.
M. genitalium-
specific serum antibodies targeting the immunodominant MgpB and MgpC proteins appeared within 1 week in three animals inoculated both cervically and in salpingeal pockets and in one of three persistently infected animals inoculated only in the cervix.
M. genitalium
-specific IgG, but not IgA, was detected in cervical secretions of serum antibody-positive animals, predominantly against MgpB and MgpC, but was insufficient to clear
M. genitalium
lower tract infection. Our findings further support female pig-tailed macaques as a model of
M. genitalium
infection, persistence, and immune evasion.
Funder
HHS | National Institutes of Health
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
16 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献