Affiliation:
1. Departments of Food Science and Animal Science, North Carolina State of the University of North Carolina at Raleigh, North Carolina
Abstract
Williamson
, W. T. (North Carolina State of the University of North Carolina, Raleigh), S. B.
Tove, and M. L. Speck
. Extracellular proteinase of
Streptococcus lactis
. J. Bacteriol.
87:
49–53. 1964.—
Streptococcus lactis
was shown to produce an extracellular proteolytic enzyme(s). A 120-fold purification of the proteinase was obtained from the cell-free culture medium by ammonium sulfate fractionation, calcium phosphate gel treatment, and chromatography on diethylaminoethyl cellulose. Only 32% of the activity was destroyed by heating at 98 C for 1 hr. The requirement of free sulfhydryl groups for enzyme activity was indicated by inhibition with
p
-chloromercuric benzoate. Diisopropyl fluorophosphate did not inhibit the proteinase.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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