Discrimination of Burkholderia multivorans and Burkholderia vietnamiensis from Burkholderia cepacia Genomovars I, III, and IV by PCR

Author:

Bauernfeind Adolf1,Schneider Ines1,Jungwirth Renate1,Roller Carsten1

Affiliation:

1. Max von Pettenkofer-Institut, Munich, Germany

Abstract

ABSTRACT We present a PCR procedure for identification of Burkholderia cepacia , Burkholderia multivorans , and Burkholderia vietnamiensis . 16S and 23S ribosomal DNAs (rDNAs) of B. multivorans and B. vietnamiensis were sequenced and aligned with published sequences for definition of species-specific 18-mer oligonucleotide primers. Specific antisense 16S rDNA primers (for B. cepacia , 5′-AGC ACT CCC RCC TCT CAG-3′; for B. multivorans , 5′-AGC ACT CCC GAA TCT CTT-3′) and 23S rDNA primers (for B. vietnamiensis , 5′-TCC TAC CAT GCG TGC AA-3′) were paired with a general sense primer of 16S rDNAs (5′-AGR GTT YGA TYM TGG CTC AG-3′) or with a sense primer of 23S rDNA (5′-CCT TTG GGT CAT CCT GGA-3′). PCR with these primers under optimized conditions is appropriate to specifically and rapidly identify B. multivorans , B. vietnamiensis , and B. cepacia (genomovars I, III, and IV are not discriminated). In comparison with the polyphasic taxonomic analyses presently necessary for species and genomovar identification within the B. cepacia complex, our procedure is more rapid and easier to perform and may contribute to clarifying the clinical significance of individual members of the complex in cystic fibrosis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference38 articles.

1. Cystic fibrosis of pancreas and its relation to celiac disease: clinical and pathological study;Andersen D. H.;Am. J. Dis. Child.,1938

2. Bauernfeind A. Jungwirth R. Schneider I. Comparative susceptibility of Burkholderia cepacia and the new species Burkholderia multivorans to ciprofloxacin ceftazidime and meropenem abstr. E-163 Abstracts of the 38th Interscience Conference on Antimicrobial Agents and Chemotherapy. 1998 216 American Society for Microbiology Washington D.C

3. Bauernfeind A. Jungwirth R. Schneider I. Ratjen F. Activity of new fluoroquinolones against Burkholderia cepacia and Burkholderia multivorans from cystic fibrosis patients abstr. 420 12th Annual North American Cystic Fibrosis Conference Montreal Canada. 1998 322 Wiley-Liss Inc. Somerset N.J

4. Microbiological background for anti-Pseudomonas aeruginosa vaccination in cystic fibrosis;Bauernfeind A.;Behring Inst. Mitt.,1997

5. Molecular Procedure for Rapid Detection of Burkholderia mallei and Burkholderia pseudomallei

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3