Molecular Procedure for Rapid Detection of Burkholderia mallei and Burkholderia pseudomallei

Author:

Bauernfeind Adolf1,Roller Carsten1,Meyer Detlef1,Jungwirth Renate1,Schneider Ines1

Affiliation:

1. Max von Pettenkofer Institut, D-80336 Munich, Germany

Abstract

ABSTRACT A PCR procedure for the discrimination of Burkholderia mallei and Burkholderia pseudomallei was developed. It is based on the nucleotide difference T 2143 C (T versus C at position 2143) between B. mallei and B. pseudomallei detected in the 23S rDNA sequences. In comparison with conventional methods the procedure allows more rapid identification at reduced risk for infection of laboratory personnel.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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