Observing Growth and Division of Large Numbers of Individual Bacteria by Image Analysis-Reference-Cited by-同舟云学术

Observing Growth and Division of Large Numbers of Individual Bacteria by Image Analysis

Published:2004-02 Issue:2 Volume:70 Page:675-678
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Elfwing A.¹,LeMarc Y.²,Baranyi J.²,Ballagi A.¹

Affiliation:

1. Center for Surface Biotechnology, University of Uppsala, S-751 23 Uppsala, Sweden

2. Institute of Food Research, Colney, Norwich NR4 7UA, United Kingdom

Abstract

ABSTRACT We describe a method that enabled us to observe large numbers of individual bacterial cells during a long period of cell growth and proliferation. We designed a flow chamber in which the cells attached to a transparent solid surface. The flow chamber was mounted on a microscope equipped with a digital camera. The shear force of the flow removed the daughter cells, making it possible to monitor the consecutive divisions of a single cell. In this way, kinetic parameters and their distributions, as well as some physiological characteristics of the bacteria, could be analyzed based on more than 1,000 single-cell observations. The method which we developed enabled us to study the history effect on the distribution of the lag times of single cells.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.2.675-678.2004

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