Superoxide Dismutase B Gene ( sodB )-Deficient Mutants of Francisella tularensis Demonstrate Hypersensitivity to Oxidative Stress and Attenuated Virulence-Reference-Cited by-同舟云学术

Superoxide Dismutase B Gene ( sodB )-Deficient Mutants of Francisella tularensis Demonstrate Hypersensitivity to Oxidative Stress and Attenuated Virulence

Published:2006-09 Issue:17 Volume:188 Page:6443-6448
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Bakshi Chandra Shekhar¹,Malik Meenakshi¹,Regan Kevin¹,Melendez J. Andres¹,Metzger Dennis W.¹,Pavlov Vitaly M.²,Sellati Timothy J.¹

Affiliation:

1. Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208

2. State Research Center for Applied Microbiology, Obolensk, Russia

Abstract

ABSTRACT A Francisella tularensis live vaccine strain mutant ( sodB Ft ) with reduced Fe-superoxide dismutase gene expression was generated and found to exhibit decreased sodB activity and increased sensitivity to redox cycling compounds compared to wild-type bacteria. The sodB Ft mutant also was significantly attenuated for virulence in mice. Thus, this study has identified sodB as an important F. tularensis virulence factor.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00266-06

Reference31 articles.

1. Anthony, L. S. D., P. J. Morrissey, and F. E. Nano. 1992. Growth inhibition of Francisella tularensis live vaccine strain by IFN-gamma-activated macrophages is mediated by reactive nitrogen intermediates derived from l-arginine metabolism. J. Immunol. 148 : 1829-1834.

2. Increased Expression of Periplasmic Cu,Zn Superoxide Dismutase Enhances Survival of Escherichia coli Invasive Strains within Nonphagocytic Cells

3. Beauchamp, C., and I. Fridovich. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44 : 276-287.

4. Carlioz, A., and D. Touati. 1986. Isolation of superoxide dismutase mutants in Escherichia coli: is superoxide dismutase necessary for aerobic life? EMBO J. 5 : 623-630.

5. Host-Pathogen Interactions: Redefining the Basic Concepts of Virulence and Pathogenicity

Cited by 95 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Deciphering Microbial Regulation of Heterogeneity and Drug Adaptation with Meta-transcriptomics and Other System-Level Tools;Antimicrobial Resistance: Factors to Findings;2024

2. Pathogenicity and virulence of Francisella tularensis;Virulence;2023-11-08

3. Strigolactones GR-24 and Nijmegen Applications Result in Reduced Susceptibility of Tobacco and Grapevine Plantlets to Botrytis cinerea Infection;Plants;2023-09-07

4. Why is manganese so valuable to bacterial pathogens?;Frontiers in Cellular and Infection Microbiology;2023-02-03

5. Critical Effect of H ₂ O ₂ in the Agar Plate on the Growth of Laboratory and Environmental Strains;Microbiology Spectrum;2022-12-21