Affiliation:
1. Servicio de Microbiología, Hospital Donostia Paseo Dr. Beguiristain s/n, 20014 San Sebastián, Spain
2. Departamento de Medicina Preventiva y Salud Pública, Universidad del País Vasco, Paseo Dr. Beguiristain s/n, 20014 San Sebastián, Spain
Abstract
ABSTRACT
The macrolide resistance determinants and genetic elements carrying the
mef
(A) and
mef
(E) subclasses of the
mef
gene were studied with
Streptococcus agalactiae
isolated in 2003 and 2004 from 7,084 vaginorectal cultures performed to detect carrier pregnant women. The prevalence of carriage was 18% (1,276 isolates), and that of erythromycin resistance 11.0% (129 of the 1,171 isolates studied).
erm
(B),
erm
(A) subclass
erm
(TR), and the
mef
gene, either subclass
mef
(A) or
mef
(E), were found in 72 (55.8%), 41 (31.8%), and 12 (9.3%) erythromycin-resistant isolates, while 4 isolates had more than 1 erythromycin resistance gene. Of the 13 M-phenotype
mef
-containing erythromycin-resistant
S. agalactiae
isolates, 11 had the
mef
(E) subclass gene alone, one had both the
mef
(E) and the
erm
(TR) subclass genes, and one had the
mef
(A) subclass gene.
mef
(E) subclass genes were associated with the carrying element mega in 10 of the 12
mef
(E)-containing strains, while the single
mef
(A) subclass gene found was associated with the genetic element Tn
1207.3
. The nonconjugative nature of the mega element and the clonal diversity of
mef
(E)-containing strains determined by pulsed-field gel electrophoresis suggest that transformation is the main mechanism through which this resistance gene is acquired.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
45 articles.
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