Rapid Pulsed-Field Gel Electrophoresis Method for Group B Streptococcus Isolates-Reference-Cited by-同舟云学术

Rapid Pulsed-Field Gel Electrophoresis Method for Group B Streptococcus Isolates

Published:2001-08 Issue:8 Volume:39 Page:3006-3008
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Benson Jeffrey A.¹,Ferrieri Patricia¹

Affiliation:

1. Departments of Laboratory Medicine and Pathology and Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota 55455

Abstract

ABSTRACT We developed a rapid pulsed-field gel electrophoresis (PFGE) method that required 3 days to complete, an improvement over the standard method that required as many as 8 days. The accuracy and reproducibility of the rapid method were verified by analysis of DNA band sizes of our control group B streptococcus isolate. The rapid method was superior to the standard method, providing more precise molecular sizing and gels of higher image quality. The reproducibility of rapid PFGE substantiated its value and continued use.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.8.3006-3008.2001

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