EPC1/TIP60-Mediated Histone Acetylation Facilitates Spermiogenesis in Mice-Reference-Cited by-同舟云学术

EPC1/TIP60-Mediated Histone Acetylation Facilitates Spermiogenesis in Mice

Published:2017-07-10 Issue:19 Volume:37 Page:
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Dong Yixin¹²,Isono Kyo-ichi¹²,Ohbo Kazuyuki³,Endo Takaho A.¹,Ohara Osamu¹,Maekawa Mamiko⁴,Toyama Yoshiro⁴,Ito Chizuru⁴,Toshimori Kiyotaka⁴,Helin Kristian⁵,Ogonuki Narumi⁶,Inoue Kimiko⁶,Ogura Atsuo⁶,Yamagata Kazutsune⁷,Kitabayashi Issay⁷,Koseki Haruhiko¹²

Affiliation:

1. RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan

2. CREST, Japan Science and Technology Agency, Yokohama, Japan

3. Department of Histology and Cell Biology, Yokohama City University School of Medicine, Yokohama, Japan

4. Department of Reproductive Biology and Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan

5. Biotech Research and Innovation Center and Center for Epigenetics, University of Copenhagen, Copenhagen, Denmark

6. RIKEN BioResource Center, Ibaraki, Japan

7. Division of Hematological Malignancy, National Cancer Center Research Institute, Tokyo, Japan

Abstract

ABSTRACT Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation-mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development. Taking these observations together, we reveal an essential role of the NuA4 complexes for histone hyperacetylation and subsequent compaction of the spermatid genome.

Funder

grants in aid for scientific research A

grant-in-aid for scientific research C

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Reference43 articles.

1. DNA and protein content of mouse sperm. Implications regarding sperm chromatin structure;Pogany;Exp Cell Res,1981

2. Molecular mechanisms of male germ cell differentiation;Hecht;Bioessays,1998

3. Haploid spermatids exhibit translationally repressed mRNAs;Steger;Anat Embryol (Berl),2001

4. Roles of transition nuclear proteins in spermiogenesis;Meistrich;Chromosoma,2003

5. Histone H1 and the origin of protamines;Lewis;Proc Natl Acad Sci U S A,2004

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