Photoresponsive Flagellum-Independent Motility of the Purple Phototrophic Bacterium Rhodobacter capsulatus

Abstract

ABSTRACT We report the discovery of photoresponsive, flagellum-independent motility of the α-proteobacterium Rhodobacter capsulatus , a nonsulfur purple phototrophic bacterium. This motility takes place in the 1.5% agar-glass interface of petri plates but not in soft agar, and cells move toward a light source. The appearances of motility assay plates inoculated with wild-type or flagellum-deficient mutants indicate differential contributions from flagellar and flagellum-independent mechanisms. Electron microscopy confirmed the absence of flagella in flagellar mutants and revealed the presence of pilus-like structures at one pole of wild-type and mutant cells. We suggest that R. capsulatus utilizes a flagellum-independent, photoresponsive mechanism that resembles twitching motility to move in a line away from the point of inoculation toward a light source.