Characterization of a Fission Yeast SUMO-1 Homologue, Pmt3p, Required for Multiple Nuclear Events, Including the Control of Telomere Length and Chromosome Segregation

Author:

Tanaka Katsunori1,Nishide Junko1,Okazaki Koei2,Kato Hiroaki1,Niwa Osami2,Nakagawa Tsuyoshi3,Matsuda Hideyuki1,Kawamukai Makoto1,Murakami Yota4

Affiliation:

1. Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, 1 and

2. Kazusa DNA Research Institute, Kisarazu 292-0812, 2 and

3. Research Institute of Molecular Genetics, 3 Shimane University, Matsue 690-8504,

4. Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto 606-0857, 4 Japan

Abstract

ABSTRACT Unlike ubiquitin, the ubiquitin-like protein modifier SUMO-1 and its budding yeast homologue Smt3p have been shown to be more important for posttranslational protein modification than for protein degradation. Here we describe the identification of the SUMO-1 homologue of fission yeast, which we show to be required for a number of nuclear events including the control of telomere length and chromosome segregation. A disruption of the pmt3 + gene, the Schizosaccharomyces pombe homologue of SMT3 , was not lethal, but mutant cells carrying the disrupted gene grew more slowly. The pmt3 Δ cells showed various phenotypes such as aberrant mitosis, sensitivity to various reagents, and high-frequency loss of minichromosomes. Interestingly, we found that pmt3 + is required for telomere length maintenance. Loss of Pmt3p function caused a striking increase in telomere length. When Pmt3p synthesis was restored, the telomeres became gradually shorter. This is the first demonstration of involvement of one of the Smt3p/SUMO-1 family proteins in telomere length maintenance. Fusion of Pmt3p to green fluorescent protein (GFP) showed that Pmt3p was predominantly localized as intense spots in the nucleus. One of the spots was shown to correspond to the spindle pole body (SPB). During prometaphase- and metaphase, the bright GFP signals at the SPB disappeared. These observations suggest that Pmt3p is required for kinetochore and/or SPB functions involved in chromosome segregation. The multiple functions of Pmt3p described here suggest that several nuclear proteins are regulated by Pmt3p conjugation.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

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