A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes-Reference-Cited by-同舟云学术

A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes

Published:2019-10-10 Issue:41 Volume:8 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Altaib Hend¹,Ozaki Yuka²,Kozakai Tomoya¹,Badr Yassien²³,Nomura Izumi²,Suzuki Tohru¹²^ORCID

Affiliation:

1. The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan

2. Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan

3. Department of Animal Medicine, Faculty of Veterinary Medicine, Damanhour University, El-Beheira, Egypt

Abstract

A series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene cloning.

Funder

MEXT | Japan Society for the Promotion of Science

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MRA.00323-19

Reference9 articles.

1. New high-cloning-efficiency vectors for complementation studies and recombinant protein overproduction in Escherichia coli and Salmonella enterica

2. Impact of targeted vector design on ColE1 plasmid replication

3. Seamless cloning and gene fusion