<i>Bifidobacterium</i> -Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in <i>Bifidobacterium</i>-Reference-Cited by-同舟云学术

Bifidobacterium -Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium

Published:2022-01-20 Issue:1 Volume:11 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Altaib Hend¹²,Ozaki Yuka²,Kozakai Tomoya¹,Sakaguchi Kouta¹,Nomura Izumi²,Suzuki Tohru¹²^ORCID

Affiliation:

1. The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan

2. Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan

Abstract

A series of Bifidobacterium - Escherichia coli shuttle vectors (pKO403- lacZ′ -Cm, pKO403- lacZ′ -Sp, pKO403- lacZ′ -p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the lacZ′ α fragment, overhung by two facing type IIS restriction sites, for blue-white selection and seamless gene cloning.

Funder

MEXT | Japan Society for the Promotion of Science

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/mra.00884-21

Reference10 articles.

1. Progress and Prospects: The design and production of plasmid vectors

2. Seamless cloning and gene fusion

3. High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening

4. Improvement of bacterial transformation efficiency using plasmid artificial modification

5. A targeted gene knockout method using a newly constructed temperature-sensitive plasmid mediated homologous recombination in Bifidobacterium longum

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