Legume- Rhizobium Interactions: Cowpea Root Exudate Elicits Faster Nodulation Response by Rhizobium Species

Author:

Bhagwat Arvind A.1,Thomas Joseph1

Affiliation:

1. Biology and Agriculture Division, Bhabha Atomic Research Centre, Trombay, Bombay 400 085, India

Abstract

Preinfection events in legume- Rhizobium symbiosis were analyzed by studying the different nodulation behaviors of two rhizobial strains in cowpeas (Vigna sinensis). Log-phase cultures of Rhizobium sp. strain 1001, an isolate from the plant nodule, initiated host responses leading to infection within 2 h after inoculation, whereas log-phase cultures of Rhizobium sp. strain 32H1 took at least 7 h to trigger a discernible response. The delay observed with strain 32H1 could be eliminated by incubating the rhizobial suspension, before inoculation, for 4.5 h either in the cowpea rhizosphere/rhizoplane condition or in the root exudate of cowpea plants, grown without NH 4 + in the rooting medium. The delay could not be eliminated by incubating the rhizobial suspension in the rooting medium of plants grown in the presence of 5 mM NH 4 + , indicating that there is a regulatory role of combined nitrogen in triggering preinfection events by the legume. The substance(s) in the root exudate which elicited the faster nodulation response by Rhizobium sp. strain 32H1 could be separated into a high-molecular-weight fraction by Sephadex G-100 gel filtration. The data support the notion that legume roots release substances that favor the development of rhizobial features essential for infection and nodulation.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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1. Biological Nitrogen Fixation;Soil Microbiology;2020-09-30

2. The Role of Flavonoids in Nodulation Host-Range Specificity: An Update;Plants;2016-08-11

3. Soil allelochemical residue effects in a tomato cowpea rotation – nodulation and productivity of cowpea and nematode suppression;Acta Agriculturae Scandinavica, Section B — Soil & Plant Science;2014-05-19

4. Bacteria in the Management of Plant-Parasitic Nematodes;Bacteria in Agrobiology: Disease Management;2012-11-30

5. Effect of legume seed exudates on the formation of Rhizobium-legume symbiosis;Applied Biochemistry and Microbiology;2009-05

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