Effect of Brilliant Green on the Motility of Salmonellae and Other Selected Microorganisms

Author:

Sessoms A. R.1,Banwart G. J.1

Affiliation:

1. Market Quality Research Division, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705

Abstract

The effect of Brilliant Green on motility was studied with Salmonella anatum, S. derby, S. tennessee, Escherichia coli, Proteus vulgaris , and Pseudomonas aeruginosa . Semisolid tryptic soy-agars containing 0, 20, or 40 mg of Brilliant Green per liter were used as the motility media. Both concentrations of Brilliant Green inhibited the growth of the non- Salmonella species through the semisolid agar. For 24 hr, the Brilliant Green appeared to limit the growth of the salmonellae; however, by 48 hr the salmonellae were able to grow through the semisolid agars. The presence of Brilliant Green in the motility media aided in the detection of Salmonella when mixed cultures were used.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference6 articles.

1. American Public Health Association. 1965. Standard methods for the examination of water and waste water 11th ed.

2. Glassware apparatus for detecting motile bacteria;Banwart G. J.;L. Salmonella. Poultry Sci.,1968

3. Multiple range and multiple F tests;Duncan D. B.;Biometrics,1955

4. Effect of various concentrations of brilliant green and bile salts on salmonellae and other microoroganisms;Miller R. B.;Appl. Microbiol.,1965

5. Brilliant green and its use in an enrichment medium in the isolation of typhoid and paratyphoid organisms;Rakieten M. L.;J. Infec. Dis.,1927

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