Author:
Koga T,Nishihara T,Fujiwara T,Nisizawa T,Okahashi N,Noguchi T,Hamada S
Abstract
Lipopolysaccharides (LPSs) were isolated from Bacteroides gingivalis and Escherichia coli by the phenol-water and butanol-water procedures. The phenol-water-extracted LPS from B. gingivalis 381 was composed of 46% carbohydrate, 23% hexosamine, 18% fatty acid, and 5% protein. The major component sugars of this preparation were glucose, glucosamine, rhamnose, galactose, galactosamine, and mannose, and their molecular ratio was 1:0.9:0.7:0.6:0.6:0.4, respectively. Neither heptose nor 2-keto-3-deoxyoctonate was detected. The butanol-water-extracted LPS from this strain was composed of 76% glucose, 7% fatty acid, and 13% protein, and it was associated with a number of polypeptides (13 to 56 kilodaltons). The main fatty acid of both LPS preparations was palmitic acid. It was found that biological activities of LPS from B. gingivalis were comparable to those of LPS from E. coli in terms of activation of the clotting enzyme of Limulus amebocyte lysate, mitogenicity, polyclonal B cell activation, and stimulation of interleukin 1 production in BALB/c mice. Furthermore, LPS-nonresponsive C3H/HeJ spleen cells were found to yield good mitogenic responses to both phenol-water-extracted LPS and butanol-water-extracted LPS from B. gingivalis or butanol-water-extracted LPS from E. coli. On the other hand, spleen cells from LPS-responsive C3H/HeN mice responded well to all these LPS preparations.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference36 articles.
1. Further improvements in the plaque technique for detecting single antibody-forming cells;Cunningham A. J.;Immunology,1968
2. Immunoelectron microscopic localization of lipopolysaccharides in the cell wall of Bacteroides oralis and Fusobacterium nucleatuim;Dahlen G.;Infect. Immun.,1978
3. Interleukin-1;Dinarello C. A.;Rev. Infect. Dis.,1984
4. Characterization of lipopolysaccharide of Haemophililuis inflienzae;Flesher A. R.;J. Infect. Dis.,1978
5. Galanos C. 0. Luderitz E. T. Rietschel and 0. Westphal. 1977. Newer aspects of the chemistry and biology of bacterial lipolysaccharides with special reference to their lipid A component p. 239-335. In T. W. Goodwin (ed.) International review of biochemistry vol. 14. University Park Press Baltimore.
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