A Novel Real-Time PCR-Based Screening Test with Pooled Fecal Samples for Bovine Johne’s Disease

Author:

Kawaji Satoko1ORCID,Nagata Reiko1,Minegishi Yasutaka2,Saruyama Yumi3,Mita Akiko4,Kishizuka Shingo4,Saito Masahiro4,Mori Yasuyuki1

Affiliation:

1. Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Japan

2. Nippon Gene Co., Ltd., Tokyo, Japan

3. Tochigi Prefectural Central Livestock Hygiene Service Center, Utsunomiya, Japan

4. National Livestock Breeding Center, Nishigo, Japan

Abstract

Johne’s disease (JD) is an economically important infectious disease in livestock farming caused by Mycobacterium avium subsp. paratuberculosis . As an alternative to serological tests, which are used mainly for the screening of whole herds, we developed a novel ResoLight-based real-time PCR (RL-PCR) assay with pooled fecal samples for the detection of fecal shedders in cattle herds. The RL-PCR assay included an internal amplification control (IC) which was amplified using the same primer pair as the target molecule M. avium subsp.

Funder

NARO | Bio-oriented Technology Research Advancement Institution

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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