Affiliation:
1. Department of Pathology, the Johns Hopkins Medical Institutions, Baltimore, Maryland
Abstract
ABSTRACT
Candida albicans
and
Candida glabrata
can be identified in blood culture bottles within 2.5 h using peptide nucleic acid fluorescence in situ hybridization. A 1.25-h protocol was compared to the standard with 40 positive (clinical and spiked) blood culture bottles tested in batches of 5. All
C. albicans
(15) and
C. glabrata
(16) isolates, alone or mixed, were identified correctly using both protocols, whereas 18 isolates (five other species) were negative by both protocols. This shortened method will significantly reduce the time to identification.
Publisher
American Society for Microbiology
Reference10 articles.
1. Büchner, T., W. Fegeler, H. Bernhardt, N. Brockmeyer, K.-H. Duswald, M. Herrmann, D. Heuser, U. Jehn, G. Just-Nübling, M. Karthaus, G. Maschmeyer, F.-M. Müller, J. Ritter, N. Roos, M. Ruhnke, A. Schmalreck, R. Schwarze, G. Schwesinger, and G. Silling. 2002. Treatment of severe Candida infections in high-risk patients in Germany: consensus formed by a panel of interdisciplinary investigators. Eur. J. Clin. Microbiol. Infect. Dis.21:337-352.
2. Use of a Commercial Reagent Leads to Reduced Germ Tube Production by
Candida dubliniensis
3. Candida albicans colony identification in 5 minutes in a general microbiology laboratory
4. Freydiere, A. M., R. Guinet, and P. Boiron. 2001. Yeast identification in the clinical microbiology laboratory: phenotypic methods. Med. Mycol.39:9-33.
5. Hazen, K. C., and S. A. Howell. 2007. Candida, Cryptococcus, and other yeasts of medical importance, p. 1762-1788. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry, and M. A. Pfaller (ed.), Manual of clinical microbiology, 9th ed., vol. 2. ASM Press, Washington, DC.
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