Affiliation:
1. National Animal Disease Center, U.S. Department of Agriculture, Ames, Iowa 50010.
Abstract
The Escherichia coli heat-stable enterotoxin STb is the most prevalent toxin associated with diarrheagenic isolates of porcine origin. This report demonstrates that when endogenous protease activity was blocked with soybean trypsin inhibitor, STb evoked a dose-dependent secretory response in infant mice and jejunal loops of rats. Infant mice were much less sensitive to STb than rats were. The response of rat jejunal loops to STb was linearly related to the log of the dose of STb through 10 twofold dilutions. The anterior 25% of the jejunum was less sensitive than was the remainder of the gut. An incubation period of 2.5 h provided a maximal response. The linear response to STb in rats could be used as a bioassay.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference19 articles.
1. Burgess M. N. R. J. Bywater C. M. Cowley N. A. Mullan and P. M. Newsome. 1978. Biological evaluation of a methanol soluble heat-stable Escherichia coli enterotoxin in infant mice pigs rabbits and calves.
2. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu;Dean A. G.;J. Infect. Dis.,1972
3. Guerrant R. L. and C. S. Weikel. 1988. Pharmacologic pathways leading to intestinal secretion: stimulation by enterotoxins and by C-kinase activators p. 181-188. In S. Kuwahara and N. F. Pierce (ed.) Advances in research on cholera and related diarrheas vol. 4. KTK Scientific Publishers Tokyo.
4. Limitations of the infant mouse test for Escherichia coli heat-stable enterotoxin (ST);Gyles C. L.;Can. J. Comp. Med.,1979
5. Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II;Handl C.;J. Clin. Microbiol.,1988
Cited by
50 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献