Quantification of Human Immunodeficiency Virus Type 1 Proviral DNA by Using TaqMan Technology-Reference-Cited by-同舟云学术

Quantification of Human Immunodeficiency Virus Type 1 Proviral DNA by Using TaqMan Technology

Published:2002-02 Issue:2 Volume:40 Page:675-678
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Zhao Yuqi¹²³⁴,Yu Min¹,Miller Johann W.²,Chen Mingzhong¹,Bremer Eric G.¹⁵,Kabat William¹,Yogev Ram¹³

Affiliation:

1. Children's Memorial Institute for Education and Research

2. Center for Biotechnology, Northwestern University

3. Departments of Pediatrics,

4. Microbiology-Immunology

5. Neurosurgery Northwestern University Medical School, Chicago, Illinois

Abstract

ABSTRACT A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.2.675-678.2002

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