The F423Y Mutation in thepfmdr2Gene and Mutations N51I, C59R, and S108N in thepfdhfrGene Are Independently Associated with Pyrimethamine Resistance in Plasmodium falciparum Isolates

Abstract

ABSTRACTScreening forin vitrosusceptibility to pyrimethamine and sequencing of thepfmdr2andpfdhfrgenes were performed in 140Plasmodium falciparumisolates. The risk ofin vitroresistance to pyrimethamine was analyzed with a logistic regression model. The mutation F423Y inpfmdr2(odds ratio [OR] = 2.12 [confidence interval {CI}, 1.02 to 4.59];P= 0.0489) and the mutation N51I, C59R, or S108N inpfdhfr(OR = 42.34 [CI, 5.52 to 324.61];P= 0.0003) were independently associated within vitroresistance to pyrimethamine.