Some Unique Properties of the Deoxyribonucleic Acid-Bearing Portion of the Bacterial Membrane-Reference-Cited by-同舟云学术

Some Unique Properties of the Deoxyribonucleic Acid-Bearing Portion of the Bacterial Membrane

Published:1972-10 Issue:1 Volume:112 Page:195-199
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Ballesta J. P.¹,Cundliffe E.¹,Daniels M. J.¹,Silverstein Judith L.¹,Susskind Miriam M.¹,Schaechter M.¹

Affiliation:

1. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, and Department of Biochemistry, Oxford University, Oxford, OX1 3QU, England

Abstract

By using the M-band technique we have shown that portions of the membranes of Bacillus megaterium and Escherichia coli vary in their affinity for magnesium-Sarkosyl crystals and in phospholipid composition. The portion to which deoxyribonucleic acid is attached comprises as little as 4% of the total cell membrane, has a particularly high degree of affinity for magnesium-Sarkosyl crystals, and is rich in phosphatidylethanolamine. The M-band fractionation does not depend on the use of lysozyme.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.112.1.195-199.1972

Reference14 articles.

1. Effect of shiftdown and growth inhibition on phospholipid metabolism of Escherichia coli;Ballesta J. P.;J. Bacteriol.,1971

2. Intracellular distribution of ribosomes and polyribosomes in Bacillus megaterium;Cundliffe E.;J. Mol. Biol.,1970

3. Some features of the bacterial membrane studied with the aid of new fractionation technique;Daniels M. J.;Biochem. J.,1971

4. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples;Dawson R. M.;Arch. Biochem. Biophys.,1960

5. DNA replication studied by a new method for the isolation of cell membrane-DNA complexes;Earhart C. F.;Cold Spring Harbor Symp. Quant. Biol.,1968

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