Development of an Efficient In Vivo System (P junc -TpaseIS 1223 ) for Random Transposon Mutagenesis of Lactobacillus casei-Reference-Cited by-同舟云学术

Development of an Efficient In Vivo System (P junc -TpaseIS 1223 ) for Random Transposon Mutagenesis of Lactobacillus casei

Published:2012-08 Issue:15 Volume:78 Page:5417-5423
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Licandro-Seraut Hélène¹²,Brinster Sophie³⁴,van de Guchte Maarten³⁴,Scornec Hélène¹,Maguin Emmanuelle³⁴,Sansonetti Philippe²,Cavin Jean-François¹,Serror Pascale³⁴

Affiliation:

1. UMR PAM, AgroSup Dijon et Université de Bourgogne, Dijon, France

2. Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, France

3. INRA, UMR1319 Micalis, Jouy-en-Josas, France

4. AgroParisTech, UMR Micalis, Jouy-en-Josas, France

Abstract

ABSTRACT The random transposon mutagenesis system P junc -TpaseIS 1223 is composed of plasmids pVI129, expressing IS 1223 transposase, and pVI110, a suicide transposon plasmid carrying the P junc sequence, the substrate of the IS 1223 transposase. This system is particularly efficient in Lactobacillus casei , as more than 10,000 stable, random mutants were routinely obtained via electroporation.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00531-12

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