A Bioinformatics Whole-Genome Sequencing Workflow for Clinical Mycobacterium tuberculosis Complex Isolate Analysis, Validated Using a Reference Collection Extensively Characterized with Conventional Methods and In Silico Approaches-Reference-Cited by-同舟云学术

A Bioinformatics Whole-Genome Sequencing Workflow for Clinical Mycobacterium tuberculosis Complex Isolate Analysis, Validated Using a Reference Collection Extensively Characterized with Conventional Methods and In Silico Approaches

Published:2021-05-19 Issue:6 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bogaerts Bert¹²^ORCID,Delcourt Thomas¹,Soetaert Karine³,Boarbi Samira³,Ceyssens Pieter-Jan³,Winand Raf¹,Van Braekel Julien¹,De Keersmaecker Sigrid C. J.¹,Roosens Nancy H. C.¹,Marchal Kathleen⁴²⁵,Mathys Vanessa³,Vanneste Kevin¹

Affiliation:

1. Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium

2. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium

3. Bacterial Diseases, Sciensano, Brussels, Belgium

4. Department of Information Technology, Internet Technology and Data Science Lab (IDLab), Interuniversity Microelectronics Centre (IMEC), Ghent University, Ghent, Belgium

5. Department of Genetics, University of Pretoria, Pretoria, South Africa

Abstract

The use of whole-genome sequencing (WGS) for routine typing of bacterial isolates has increased substantially in recent years. For Mycobacterium tuberculosis (MTB), in particular, WGS has the benefit of drastically reducing the time required to generate results compared to most conventional phenotypic methods.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00202-21

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