Use of Rotavirus Virus-Like Particles as Surrogates To Evaluate Virus Persistence in Shellfish-Reference-Cited by-同舟云学术

Use of Rotavirus Virus-Like Particles as Surrogates To Evaluate Virus Persistence in Shellfish

Published:2005-10 Issue:10 Volume:71 Page:6049-6053
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Loisy Fabienne¹,Atmar Robert L.²,Le Saux Jean-Claude¹,Cohen Jean³,Caprais Marie-Paule¹,Pommepuy Monique¹,Le Guyader Françoise S.¹

Affiliation:

1. Laboratoire de Microbiologie, IFREMER, BP 21 105, 44311 Nantes cedex 03, France

2. Department of Molecular Virology and Microbiology, Baylor College of Medicine, 1 Baylor Plaza, Houston, Texas 77030

3. Virologie Moleculaire et Structurale, Unité mixte CNRS-INRA, 1 av. de la Terrasse, 91198 Gif-sur-Yvette, France

Abstract

ABSTRACT Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log 10 of VLPs was depurated after 1 week in warm seawater (22°C). Phage MS2 was depurated more rapidly (about 2 log 10 in 1 week) than were VLPs, as determined using a single-compartment model and linear regression analysis. After being relayed in the estuary under the influence of the tides, VLPs were detected in oysters for up to 82 days following seeding with high levels of VLPs (concentration range between 10 10 and 10 9 particles per g of pancreatic tissue) and for 37 days for lower contamination levels (10 5 particles per g of pancreatic tissue). These data suggest that viral particles may persist in shellfish tissues for several weeks.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.10.6049-6053.2005

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