Alternative medium for Clostridium perfringens sporulation

Author:

Tórtora J C

Abstract

A medium containing 0.50 g of thiotone peptone, 0.30 g of soluble starch, 0.02 g of MgSO4 X 7H2O, 0.90 g of Na2HPO4 X 2H2O, 100.00 ml of distilled water, and optionally , 166 micrograms of dichloridric thiamine supported sporulation of 138 out of 141 Clostridium perfringens strains. Comparatively this medium gave a greater percentage of sporulation than five other media described previously.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference8 articles.

1. Quantitation of Clostridiumn perfiingens in foods;Angelotti R.;Appl. Microbiol.,1962

2. A paracrystalline inclusion formed during sporulation of enterotoxin-producing strains of Clostridilin perfringens type A;Duncan C. L.;J. Bacteriol.,1973

3. Improved medium for sporulation of Closiridiuin peifringens;Duncan C. L.;Appl. Microbiol.,1968

4. A medium promoting rapid quantitative sporulation in Clostridilun perfringens;ElIner P. D.;J. Bacteriol.,1956

5. Holdeman L. V. and W. E. C. Moore. 1972. Anaerobe laboratory manual 2nd ed. Virginia Polytechnic Institute and State University. Blacksburg.

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