Real-Time PCR as a New Tool for Quantifying Leishmania infantum in Liver in Infected Mice-Reference-Cited by-同舟云学术

Real-Time PCR as a New Tool for Quantifying Leishmania infantum in Liver in Infected Mice

Published:2001-07 Issue:4 Volume:8 Page:828-831
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Bretagne Stéphane¹,Durand Rémy²,Olivi Martine³,Garin Jean-François⁴,Sulahian Annie⁴,Rivollet Danièle¹,Vidaud Michel³,Deniau Michèle¹

Affiliation:

1. Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor-APHP and Université Paris XII, Créteil,1 and

2. Laboratoire de Parasitologie-Mycologie, Hôpital Bichat-APHP,2

3. Laboratoire de Génétique Moléculaire, Faculté de Pharmacie,3 and

4. Laboratoire de Parasitologie-Mycologie, Hôpital Saint-Louis-APHP and Université Paris VI,4 Paris, France.

Abstract

ABSTRACT The parasitic loads of mouse livers experimentally infected with Leishmania infantum were determined using a double real-time quantitative PCR test targeted to the parasite DNA polymerase gene and to the mouse brain-derived neutrophic factor gene. The Leishmania DNA copy number was normalized to the number of mouse gene copies in order to quantify the former independently of liver weight. The correlation coefficient with the microtitration method was 0.66. This PCR assay can be considered for experimental pharmaceutical studies.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.8.4.828-831.2001

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