Culture microtitration: a sensitive method for quantifying Leishmania infantum in tissues of infected mice

Author:

Buffet P A1,Sulahian A1,Garin Y J1,Nassar N1,Derouin F1

Affiliation:

1. Laboratoire de Parasitologie-Mycologie, Faculté de Médecine Lariboisière Saint-Louis, Paris, France.

Abstract

We developed a microtitration method to determine the parasite burdens in homogenized organs of mice infected with Leishmania infantum. This method proved more sensitive than direct enumeration of amastigotes in stained organs, was appropriate for describing the kinetics of infection, and can be considered for physiopathological or pharmaceutical experimental studies.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

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5. A limiting dilution assay for quantifying Leishmania major in tissues of infected mice;Titus R. G.;Parasite Immunol.,1985

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