Cofactors for Human Immunodeficiency Virus Type 1 cDNA Integration In Vitro-Reference-Cited by-同舟云学术

Cofactors for Human Immunodeficiency Virus Type 1 cDNA Integration In Vitro

Published:2003-01-15 Issue:2 Volume:77 Page:1598-1603
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Gao Kui¹,Gorelick Robert J.²,Johnson Donald G.²,Bushman Frederic¹

Affiliation:

1. Infectious Disease Laboratory, The Salk Institute, La Jolla, California 92037

2. AIDS Vaccine Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, Maryland 21702

Abstract

ABSTRACT We have investigated the function of two DNA binding proteins that stimulate human immunodeficiency virus type 1 cDNA integration in vitro, the cellular HMGa1 protein and the viral nucleocapsid (NC) protein. Of the three forms of NC (NCp7, NCp9, and NCp15), we find that NCp9 is the most effective at increasing integration in vitro; thus, processing of NC may potentially modulate its activities during infection. We also found that maximal stimulation by NCp9 required roughly enough NC to coat the reactant DNAs whereas less HMGa1 was required, and the reactions displayed different optima for divalent metal cofactors and order of addition. These findings reveal probable distinct mechanisms of action in vitro.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.77.2.1598-1603.2003

Reference35 articles.

1. Brin, E., and J. Leis. 2002. Changes in the mechanism of DNA integration in vitro induced by base substitutions in the HIV-1 U5 and U3 terminal sequences. J. Biol. Chem. 277 : 10938-10948.

2. Buckman, J. S., W. J. Bosche, and R. J. Gorelick. 2002. Human immunodeficiency virus type 1 nucleocapsid Zn2+ fingers are required for efficient reverse transcription, initial integration processes, and protection of newly synthesized viral DNA. J. Virol. 77 : 1469-1480.

3. Bukrinsky, M. I., N. Sharova, T. L. McDonald, T. Pushkarskaya, G. W. Tarpley, and M. Stevenson. 1993. Association of integrase, matrix, and reverse transcriptase antigens of human immunodeficiency virus type 1 with viral nucleic acids following acute infection. Proc. Natl. Acad. Sci. USA 90 : 6125-6129.

4. Bushman F. D. 2001. Lateral DNA transfer: mechanisms and consequences. Cold Spring Harbor Laboratory Press New York N.Y.

5. Sequence requirements for integration of Moloney murine leukemia virus DNA in vitro

Cited by 55 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. N-Substituted Quinolinonyl Diketo Acid Derivatives as HIV Integrase Strand Transfer Inhibitors and Their Activity against RNase H Function of Reverse Transcriptase;Journal of Medicinal Chemistry;2015-05-26

2. RNA-Mediated Regulation of HMGA1 Function;Biomolecules;2015-05-14

3. Novel fused tetrathiocines as antivirals that target the nucleocapsid zinc finger containing protein of the feline immunodeficiency virus (FIV) as a model of HIV infection;Bioorganic & Medicinal Chemistry Letters;2015-03

4. The HIV-1 Nucleocapsid Protein Recruits Negatively Charged Lipids To Ensure Its Optimal Binding to Lipid Membranes;Journal of Virology;2014-11-19

5. Retrospective on the all-in-one retroviral nucleocapsid protein;Virus Research;2014-11