High-Resolution Differentiation of Enteric Bacteria in Premature Infant Fecal Microbiomes Using a Novel rRNA Amplicon-Reference-Cited by-同舟云学术

High-Resolution Differentiation of Enteric Bacteria in Premature Infant Fecal Microbiomes Using a Novel rRNA Amplicon

Published:2021-02-23 Issue:1 Volume:12 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Graf J.¹^ORCID,Ledala N.²,Caimano M. J.²³,Jackson E.⁴,Gratalo D.⁴,Fasulo D.⁵,Driscoll M. D.⁴,Coleman S.²,Matson A. P.²⁶

Affiliation:

1. University of Connecticut, Department of Molecular and Cell Biology, Storrs, Connecticut, USA

2. UConn Health, Department of Pediatrics, Farmington, Connecticut, USA

3. UConn Health, Department of Medicine, Farmington, Connecticut, USA

4. Shoreline Biome, Farmington, Connecticut, USA

5. Pattern Genomics, Madison, Connecticut, USA

6. Connecticut Children’s Medical Center, Division of Neonatology, Hartford, Connecticut, USA

Abstract

Achieving strain-level resolution is a major obstacle for source tracking and temporal studies of microbiomes. In this study, we describe a novel deep-sequencing approach that provides species- and strain-level resolution of the neonatal microbiome.

Funder

Connecticut Children's

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.03656-20

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