Central Nervous System (CNS) Viral Seeding by Mature Monocytes and Potential Therapies To Reduce CNS Viral Reservoirs in the cART Era-Reference-Cited by-同舟云学术

Central Nervous System (CNS) Viral Seeding by Mature Monocytes and Potential Therapies To Reduce CNS Viral Reservoirs in the cART Era

Published:2021-04-27 Issue:2 Volume:12 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

León-Rivera Rosiris¹^ORCID,Veenstra Mike¹,Donoso Maribel²,Tell Elizabeth³,Eugenin Eliseo A.²^ORCID,Morgello Susan³⁴,Berman Joan W.¹⁵^ORCID

Affiliation:

1. Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA

2. Department of Neuroscience, Cell Biology, and Anatomy, University of Texas Medical Branch, Galveston, Texas, USA

3. Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, New York, USA

4. Departments of Pathology and Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York, USA

5. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA

Abstract

We characterized mechanisms of CNS viral reservoir establishment/replenishment using peripheral blood mononuclear cells (PBMC) of PLWH on cART and propose therapeutic targets to reduce/block selective entry of cells harboring HIV (HIV + ) into the CNS. Using DNA/RNAscope, we show that CD14 + CD16 + monocytes with integrated HIV, transcriptionally active, and/or with active viral replication from PBMC of PLWH prescribed cART and virally suppressed, selectively transmigrate across a human BBB model.

Funder

Einstein-Rockefeller-CUNY Center for AIDS Research

HHS | NIH | National Institute of Mental Health

YU | Albert Einstein College of Medicine, Yeshiva University

Mount Sinai Institute for NeuroAIDS Disparities

HHS | NIH | National Institute on Drug Abuse

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.03633-20