Affiliation:
1. Department of Microbiology and Immunology, Washington University, St. Louis, Missouri 63130.
Abstract
Restriction fragment length polymorphism analysis has been used to assess relatedness among the genomes of four isolates of Mycobacterium leprae, the causative agent of leprosy. The M. leprae isolates were from human patients from India, a Mangabey monkey from West Africa, and an armadillo from Louisiana. A total of 16 probes were used; these were insert fragments of M. leprae DNA from plasmid recombinant libraries, 5 of which had genes with identifiable functions and 11 of which were randomly chosen recombinant molecules. In spite of the widely diverse origins of the isolates, restriction fragment length polymorphism analysis demonstrated that less than 0.3% of the nucleotides differ among the genomes.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference32 articles.
1. Conservation and variation of nucleotide sequences within related bacterial genomes: Escherichia coli strains;Anilionis A.;J. Bacteriol.,1980
2. Deoxyribonucleic acid relatedness among Mycobacterium leprae, Mycobacterium tuberculosis, and selected bacteria by dot blot and spectrophotometric deoxyribonucleic acid hybridization assays;Athwal R. S.;Int. J. Syst. Bacteriol.,1984
3. Bachmann B. J. 1987. Linkage map of Escherichia coli K-12 edition 7 p. 807-876. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology vol. 2. American Society for Microbiology Washington D.C.
4. Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome;Bercovier H.;Biochem. Biophys. Res. Commun.,1986
5. Construction of a genetic linkage map in man using restriction fragment length polymorphisms;Botstein D.;Am. J. Hum. Genet.,1980
Cited by
46 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献