Cloning and Nucleotide Sequence of the DNA Gyrase gyrA Gene from Serratia marcescens and Characterization of Mutations in gyrA of Quinolone-Resistant Clinical Isolates-Reference-Cited by-同舟云学术

Cloning and Nucleotide Sequence of the DNA Gyrase gyrA Gene from Serratia marcescens and Characterization of Mutations in gyrA of Quinolone-Resistant Clinical Isolates

Published:1998-01 Issue:1 Volume:42 Page:190-193
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Kim Jeong Hoon¹,Cho Eun Hee²,Kim Kwang Seo³,Kim Hak Yeop¹,Kim Young Min³

Affiliation:

1. Department of Pharmacology and Molecular Biology, C&C Research Laboratories, Kyunggi-do 445-970,1

2. Department of Science Education, Chosun University, Kwangju 501-759,2and

3. Molecular Microbiology Laboratory, Department of Biology, Yonsei University, Seoul 120-749,3Korea

Abstract

ABSTRACT The sequence of the DNA gyrase gyrA gene of Serratia marcescens ATCC 14756 was determined. An open reading frame of 2,640 nucleotides coding for a polypeptide with a calculated molecular mass of 97,460 was found, and its sequence complemented the sequence of an Escherichia coli gyrA temperature-sensitive mutation. Analysis of the PCR products of the quinolone resistance-determining regions of gyrA genes from six quinolone-resistant clinical isolates revealed a single amino acid substitution, Ser-83 to Arg or Asp-87 to Tyr, in all six mutants, suggesting that a mutational alteration in gyrA is a common mechanism of quinolone resistance in S. marcescens.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.42.1.190

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