Novel Methylotrophy Genes of Methylobacterium extorquens AM1 Identified by using Transposon Mutagenesis Including a Putative Dihydromethanopterin Reductase-Reference-Cited by-同舟云学术

Novel Methylotrophy Genes of Methylobacterium extorquens AM1 Identified by using Transposon Mutagenesis Including a Putative Dihydromethanopterin Reductase

Published:2003-01-15 Issue:2 Volume:185 Page:669-673
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Marx Christopher J.¹,O'Brien Brooke N.¹,Breezee Jennifer¹,Lidstrom Mary E.¹²

Affiliation:

1. Departments of Microbiology

2. Chemical Engineering, University of Washington, Seattle, Washington 98195-1750

Abstract

ABSTRACT Ten novel methylotrophy genes of the facultative methylotroph Methylobacterium extorquens AM1 were identified from a transposon mutagenesis screen. One of these genes encodes a product having identity with dihydrofolate reductase (DHFR). This mutant has a C 1 -defective and methanol-sensitive phenotype that has previously only been observed for strains defective in tetrahydromethanopterin (H 4 MPT)-dependent formaldehyde oxidation. These results suggest that this gene, dmrA , may encode dihydromethanopterin reductase, an activity analogous to that of DHFR that is required for the final step of H 4 MPT biosynthesis.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.185.2.669-673.2003

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