Calculation of K m and V max from Substrate Concentration Versus Time Plot-Reference-Cited by-同舟云学术

Calculation of K m and V max from Substrate Concentration Versus Time Plot

Published:1979-10 Issue:4 Volume:38 Page:758-760
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Counotte G. H. M.¹,Prins R. A.¹

Affiliation:

1. Laboratory of Animal Nutrition, State University of Utrecht, Yalelaan 1, Utrecht, The Netherlands

Abstract

A simple method for the calculation of kinetic parameters ( K m , V max ) under conditions of changing substrate concentrations is presented. An application of the method to detect shifts in groups involved in the utilization of a substrate in a mixed microbial culture is given.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.38.4.758-760.1979

Reference2 articles.

1. Bergmeyer H. U. 1970. Methoden der Enzymatischen Analysen 2nd ed. p. 1 425. Verlag Chemie Weinheim Germany.

2. Statistical estimation in enzyme kinetics;Wilkinson G. N.;Biochem. J.,1961

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