Inducible amber suppressor for Bacillus subtilis-Reference-Cited by-同舟云学术

Inducible amber suppressor for Bacillus subtilis

Published:1994-04 Issue:7 Volume:176 Page:2108-2110
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Grundy F J¹,Henkin T M¹

Affiliation:

1. Department of Biochemistry and Molecular Biology, Albany Medical College, New York 12208.

Abstract

An amber suppressor variant of Bacillus subtilis tyrosyl-tRNA was constructed and placed under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible Pspac promoter. Addition of IPTG resulted in a 50-fold increase in the expression of an rpsD-lacZ fusion containing a UAG amber codon. This system permitted isolation of a conditional lethal mutant which required IPTG for growth.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.176.7.2108-2110.1994

Reference16 articles.

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5. The rpsD gene, encoding ribosomal protein S4, is autogenously regulated in Bacillus subtilis;Grundy F. J.;J. Bacteriol.,1991

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