Affiliation:
1. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Abstract
Multiple auxotrophic strains of
Bacillus subtilis
168 were tested for joint one-step reversion of two or more auxotrophic markers to the wild-type phenotype. Mu8u5u5, a strain requiring leucine, methionine, and threonine, yielded revertants that grew without added methionine or threonine and proved to have a suppressor gene. When transferred by transformation with deoxyribonucleic acid, this suppressor gene also suppressed the adenine mutation in another strain, Mu8u5u6. The one-step double revertants fell into two distinct classes: strains of class
su
+
I
grow well in broth; strains of class
su
+
II
grow poorly. Strains
su
+
II
tend to revert frequently to the
su
+
I
or
su
−
state. Conditional lethal mutants of phage φe were isolated which can grow on the
su
+
and not on the
su
−
strains.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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