Comparison of Methods for DNA Isolation from Food Samples for Detection of Shiga Toxin-Producing Escherichia coli by Real-Time PCR-Reference-Cited by-同舟云学术

Comparison of Methods for DNA Isolation from Food Samples for Detection of Shiga Toxin-Producing Escherichia coli by Real-Time PCR

Published:2003-03 Issue:3 Volume:69 Page:1844-1846
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Heller Loree C.¹,Davis Carisa R.²,Peak K. Kealy²,Wingfield David³,Cannons Andrew C.²,Amuso Philip T.²³,Cattani Jacqueline²

Affiliation:

1. Esoteric Testing, Department of Pathology, Tampa General Hospital, Tampa, Florida 33101

2. Center for Biological Defense, University of South Florida College of Public Health

3. Bureau of Laboratories—Tampa, Florida Department of Health, Tampa, Florida 33612

Abstract

ABSTRACT In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.69.3.1844-1846.2003

Reference8 articles.

1. Jordan J. A. 2000. Real-time detection of PCR products and microbiology. New technologies for life sciences: a trends guide. 6: 61-66.

2. Polymerase chain reaction detection of nonviable bacterial pathogens

3. Kolavic, S. A., A. Kimura, S. L. Simons, L. Slutsker, S. Barth, and C. E. Haley. 1997. An outbreak of Shigella dysenteriae type 2 among laboratory workers due to intentional food contamination. JAMA278:396-398.

4. Masters, C. I., J. A. Shallcross, and B. M. Mackey. 1994. Effect of stress treatments on the detection of Listeria monocytogenes and enterotoxigenic Escherichia coli by the polymerase chain reaction. J. Appl. Bacteriol.77:73-79.

5. Murray P. R. E. J. Baron M. A. Pfaller F. C. Tenover and R. H. Yolken (ed.). 1999. Manual of clinical microbiology 7th ed. ASM Press Washington D.C.

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