Whole-Genome Characterization of Human and Simian Immunodeficiency Virus Intrahost Diversity by Ultradeep Pyrosequencing-Reference-Cited by-同舟云学术

Whole-Genome Characterization of Human and Simian Immunodeficiency Virus Intrahost Diversity by Ultradeep Pyrosequencing

Published:2010-11-15 Issue:22 Volume:84 Page:12087-12092
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Bimber Benjamin N.¹,Dudley Dawn M.²,Lauck Michael²,Becker Ericka A.¹,Chin Emily N.²,Lank Simon M.¹,Grunenwald Haiying L.³,Caruccio Nicholas C.³,Maffitt Mark³,Wilson Nancy A.¹,Reed Jason S.¹,Sosman James M.⁴,Tarosso Leandro F.⁵,Sanabani Sabri⁵,Kallas Esper G.⁵,Hughes Austin L.⁶,O'Connor David H.¹²

Affiliation:

1. Wisconsin National Primate Research Center, University of Wisconsin—Madison, Madison, Wisconsin 53706

2. Department of Pathology and Laboratory Medicine, University of Wisconsin—Madison, Madison, Wisconsin 53706

3. Epicentre Biotechnologies, 726 Post Road, Madison, Wisconsin 53713

4. University of Wisconsin Carbone Cancer Center, Madison, Wisconsin 53705

5. Division of Clinical Immunology and Allergy, University of Sao Paulo, Sao Paulo, Brazil

6. Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208

Abstract

ABSTRACT Rapid evolution and high intrahost sequence diversity are hallmarks of human and simian immunodeficiency virus (HIV/SIV) infection. Minor viral variants have important implications for drug resistance, receptor tropism, and immune evasion. Here, we used ultradeep pyrosequencing to sequence complete HIV/SIV genomes, detecting variants present at a frequency as low as 1%. This approach provides a more complete characterization of the viral population than is possible with conventional methods, revealing low-level drug resistance and detecting previously hidden changes in the viral population. While this work applies pyrosequencing to immunodeficiency viruses, this approach could be applied to virtually any viral pathogen.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01378-10

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