Ultradeep Pyrosequencing Detects Complex Patterns of CD8 + T-Lymphocyte Escape in Simian Immunodeficiency Virus-Infected Macaques-Reference-Cited by-同舟云学术

Ultradeep Pyrosequencing Detects Complex Patterns of CD8 + T-Lymphocyte Escape in Simian Immunodeficiency Virus-Infected Macaques

Published:2009-08-15 Issue:16 Volume:83 Page:8247-8253
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Bimber Benjamin N.¹,Burwitz Benjamin J.²,O'Connor Shelby²,Detmer Ann¹,Gostick Emma³,Lank Simon M.¹,Price David A.³,Hughes Austin⁴,O'Connor David¹²

Affiliation:

1. Wisconsin National Primate Research Center, University of Wisconsin—Madison, Madison, Wisconsin 53706

2. Department of Pathology and Laboratory Medicine, University of Wisconsin—Madison, Madison, Wisconsin 53706

3. Department of Medical Biochemistry and Immunology, Cardiff University School of Medicine, Heath Park, Cardiff, United Kingdom

4. Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208

Abstract

ABSTRACT Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultradeep pyrosequencing to create a comprehensive picture of CD8 + T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a previously undetected complex pattern of viral variants. This increased sensitivity enabled the detection of acute CD8-TL escape as early as 17 days postinfection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study the evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00897-09

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