The Roles of Nicotinamide Adenine Dinucleotide Phosphate Reoxidation and Ammonium Assimilation in the Secretion of Amino Acids as Byproducts of Clostridium thermocellum-Reference-Cited by-同舟云学术

The Roles of Nicotinamide Adenine Dinucleotide Phosphate Reoxidation and Ammonium Assimilation in the Secretion of Amino Acids as Byproducts of Clostridium thermocellum

Published:2023-01-31 Issue:1 Volume:89 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Yayo Johannes¹,Rydzak Thomas²³,Kuil Teun¹,Karlsson Anna¹,Harding Dan J.⁴,Guss Adam M.²³^ORCID,van Maris Antonius J. A.¹^ORCID

Affiliation:

1. Department of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden

2. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

3. Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

4. Department of Chemical Engineering, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden

Abstract

Improving the ethanol yield of C. thermocellum is important for the industrial implementation of this microorganism in consolidated bioprocessing. A central role of NADPH in driving amino acid byproduct formation was demonstrated by eliminating the NADPH-supplying malate shunt and separately by changing the cofactor specificity in ammonium assimilation.

Funder

Svenska Forskningsrådet Formas

Novo Nordisk Foundation

Swedish Foundation for Strategic Research

DOE | SC | Center for Bioenergy Innovation

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.01753-22

Reference63 articles.