Affiliation:
1. Department of Biology, Texas A&M University, College Station, Texas 77843
Abstract
ABSTRACT
Several different environmental signals can induce asexual spore development (conidiation) and expression of developmentally regulated genes in
Neurospora crassa
. However, under constant conditions, where no environmental cues for conidiation are present, the endogenous circadian clock in
N. crassa
promotes daily rhythms in expression of known developmental genes and of conidiation. We anticipated that the same pathway of gene regulation would be followed during clock-controlled conidiation and environmental induction of conidiation and that the circadian clock would need only to control the initial developmental switch. Previous experiments showed that high-level developmental induction of the clock-controlled genes
eas
(
ccg-2
) and
ccg-1
requires the developmental regulatory proteins FL and ACON-2, respectively, and normal developmental induction of
fl
mRNA expression requires ACON-2. We demonstrate that the circadian clock regulates rhythmic
fl
gene expression and that
fl
rhythmicity requires ACON-2. However, we find that clock regulation of
eas
(
ccg-2
) is normal in an
fl
mutant strain and
ccg-1
expression is rhythmic in an
acon-2
mutant strain. Together, these data point to the endogenous clock and the environment following separate pathways to regulate conidiation-specific gene expression.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Cited by
23 articles.
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