Affiliation:
1. Glaxo Research Ltd., and Glaxo Laboratories Ltd., Greenford, Middlesex, England
Abstract
The hydrolysis of two cephalosporins by three different β-lactamases has been studied. Each enzyme caused a decrease in ultraviolet absorption, a loss of biological activity, and the release of the leaving group from the 3-position. The changes occurred at the same rate and to the same extent with each enzyme, and it is inferred that the loss of the leaving group is a consequence of, and not a prerequisite for, hydrolysis of the β-lactam ring.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference6 articles.
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5. Novel method of detection of ,B-lactamases using a chromogenic cephalosporin substrate;O'Callaghan C. H.;Antimicrob. Ag. Chemother.,1972
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