A Simple Microfluidic Assay for Diagnosing Tuberculous Meningitis in HIV-Uninfected Patients-Reference-Cited by-同舟云学术

A Simple Microfluidic Assay for Diagnosing Tuberculous Meningitis in HIV-Uninfected Patients

Published:2019-05 Issue:5 Volume:57 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Park Joung Ha¹,Jin Choong Eun²,Koo Bonhan²,Kwon Ji-Soo¹,Cha Hye Hee¹,Kim Ji Yeun¹,Noh Geun Su²,Koo Yong Seo³,Jeon Sang-Beom³,Lee Sang-Ahm³,Shin Yong²,Kim Sung-Han¹

Affiliation:

1. Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea

2. Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea

3. Department of Neurology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea

Abstract

We evaluated the diagnostic performance of a s imple and l abel-free pathogen enrichment method using homobifunctional i midoesters (HI) and a m icrofluidic system, called the SLIM assay, followed by real-time PCR from cerebrospinal fluid (CSF) in human immunodeficiency virus (HIV)-uninfected patients with suspected tuberculous meningitis (TBM). Patients with suspected TBM were prospectively enrolled in a tertiary hospital in an intermediate tuberculosis (TB)-burden country during a 30-month period.

Funder

National Research Foundation of Korea

Asan Institute for Life Sciences, Asan Medical Center

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01975-18

Reference16 articles.

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