Affiliation:
1. Department of Biotechnology, Indian Institute of Technology—Madras, Chennai, India 600036
Abstract
ABSTRACT
HtrA is a unique protease on the extracellular surface of
Lactococcus lactis
. It is known to take part in the proteolysis of many secreted recombinant proteins, and the mutation of
htrA
can lead to the complete stabilization of recombinant proteins. In this work, we have shown that
htrA
mutation also leads to significant reduction of the efficiency of recombinant-protein secretion. We also show that the level of HtrA can be lowered by the suppression of the acid tolerance response (ATR) in
L. lactis
. Instead of using an
L. lactis htrA
mutant, the reduction of the HtrA level in wild-type recombinant cultures of
L. lactis
by ATR suppression may serve as a better strategy for the production of secreted recombinant proteins.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference18 articles.
1. Bermudez-Humaran, L. G., P. Langella, J. Commissaire, S. Gilbert, Y. Le Loir, R. L'Haridon, and G. Corthier. 2003. Controlled intra- or extracellular production of staphylococcal nuclease and ovine omega interferon in Lactococcus lactis. FEMS Microbiol. Lett.224:307-313.
2. Bredmose, L., S. M. Madsen, A. Vrang, P. Ravn, M. G. Johnsen, J. Arnau, and H. Israelsen. 2001. Development of a heterologous gene expression system for use in Lactococcus lactis, p. 269-275. In O. W. Merten, D. Mattonovich, C. Lang, G. Larsson, P. Neubauer, D. Porro, P. Postma, J. T. de Mattos, and J. A. Cole (ed.), Recombinant protein production with prokaryotic and eukaryotic cells. Kluwer Academic Publishers, Dordrecht, The Netherlands.
3. Cortes-Perez, N. G., I. Poquet, M. Oliveira, J. J. Gratadoux, S. M. Madsen, A. Miyoshi, G. Corthier, V. Azevedo, P. Langella, and L. G. Bermudez-Humaran. 2006. Construction and characterization of a Lactococcus lactis strain deficient in intracellular ClpP and extracellular HtrA proteases. Microbiology152:2611-2618.
4. Turbidimetric Assay of Staphylococcal Nuclease
5. Foucaud-Scheunemann, C., and I. Poquet. 2003. HtrA is a key factor in the response to specific stress conditions in Lactococcus lactis. FEMS Microbiol. Lett.224:53-59.
Cited by
18 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献