Sequencing, Cloning, and High-Level Expression of the pfp Gene, Encoding a PP i -Dependent Phosphofructokinase from the Extremely Thermophilic Eubacterium Dictyoglomus thermophilum-Reference-Cited by-同舟云学术

Sequencing, Cloning, and High-Level Expression of the pfp Gene, Encoding a PP i -Dependent Phosphofructokinase from the Extremely Thermophilic Eubacterium Dictyoglomus thermophilum

Published:2000-08-15 Issue:16 Volume:182 Page:4661-4666
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Ding Yan-Huai R.¹,Ronimus Ron S.¹,Morgan Hugh W.¹

Affiliation:

1. Thermophile Research Unit, Department of Biological Sciences, The University of Waikato, Hamilton, New Zealand

Abstract

ABSTRACT The sequencing, cloning, and expression of the pfp gene from Dictyoglomus thermophilum , which consists of 1,041 bp and encodes a pyrophosphate-dependent phosphofructokinase, are described. A phylogenetic analysis indicates that the enzyme is closely related to the pyrophosphate-dependent enzyme from Thermoproteus tenax . The recombinant and native enzymes share a high degree of similarity for most properties examined.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.182.16.4661-4666.2000

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