Increasing the Thermodynamic Driving Force of the Phosphofructokinase Reaction in <i>Clostridium thermocellum</i>-Reference-Cited by-同舟云学术

Increasing the Thermodynamic Driving Force of the Phosphofructokinase Reaction in Clostridium thermocellum

Published:2022-11-22 Issue:22 Volume:88 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Hon Shuen¹²³^ORCID,Jacobson Tyler²⁴,Stevenson David M.²⁴,Maloney Marybeth I.¹²,Giannone Richard J.²⁵,Hettich Robert L.²⁵,Amador-Noguez Daniel²⁴^ORCID,Olson Daniel G.¹²^ORCID,Lynd Lee R.¹²³

Affiliation:

1. Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA

2. Center for Bioenergy Innovation, Oak Ridge, Tennessee, USA

3. Enchi Corporation, Hanover, New Hampshire, USA

4. Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin, USA

5. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

Abstract

The ability to control the distribution of thermodynamic driving force throughout a metabolic pathway is likely to be an important tool for metabolic engineering. The phosphofructokinase reaction is a key enzyme in Embden-Mayerhof-Parnas glycolysis and therefore improving the thermodynamic driving force of this reaction in C. thermocellum is believed to enable higher product titers.

Funder

DOE | SC | Center for Bioenergy Innovation

Joint Genome Institute

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.01258-22

Reference51 articles.

1. Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production

2. Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum