Characterization of Denitrification Gene Clusters of Soil Bacteria via a Metagenomic Approach-Reference-Cited by-同舟云学术

Characterization of Denitrification Gene Clusters of Soil Bacteria via a Metagenomic Approach

Published:2009-01-15 Issue:2 Volume:75 Page:534-537
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Demanèche Sandrine¹,Philippot Laurent²³,David Maude M.¹,Navarro Elisabeth¹⁴,Vogel Timothy M.¹,Simonet Pascal¹

Affiliation:

1. Environmental Microbial Genomics Group, Laboratoire AMPERE, UMR CNRS 5005, Ecole Centrale de Lyon, Université de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully, France

2. INRA, UMR 1229, F-21000 Dijon, France

3. University of Burgundy, UMR 1229, F-21000 Dijon, France

4. Laboratoire des Symbioses Tropicales et Méditerranéennes, UMR 113 IRD, CIRAD, AGRO Montpellier, INRA, Université Montpellier 2, TA A-82/J, Campus de Baillarguet, 34398 Montpellier Cedex 5, France

Abstract

ABSTRACT We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01706-08

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