Membrane-bound nucleotidase of Bacillus cereus

Author:

Schrader W P,Anderson J S

Abstract

A membrane-bound nucleotidase of Bacillus cereus T was solubilized by digestion with trypsin and subsequently purified more than 300-fold. The purified nucleotidase was most active on ribonucleoside 5'-monophosphates and was slightly less active (40 to 60%) on deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates. In addition to hydrolytic activity, the nucleotidase preparation possessed phosphotransferase activity by which phosphate is transferred from a phosphate donor to the 5' position of nucleosides.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Cited by 6 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Purine and Pyrimidine Salvage Pathways;Bacillus subtilis and Other Gram-Positive Bacteria;2014-04-30

2. General Enzymatic Screens Identify Three New Nucleotidases in Escherichia coli;Journal of Biological Chemistry;2004-12

3. Membrane-bound 5′-nucleotidase/nucleoside phosphotransferase from Bacillus cereus;International Journal of Biochemistry;1993-11

4. Nucleotidase;Enzyme Handbook 3;1991

5. Bacillus cereus electron transport and proton motive force during aerotaxis;Journal of Bacteriology;1984-09

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