Abstract
An isogenic set of mutants of Pseudomonas aeruginosa, altered in permeability or permeability plus constitutive production of beta-lactamase, was examined for susceptibility to newer beta-lactam antibiotics. Kinetic data on the chromosomal beta-lactamase and susceptibility studies for the test beta-lactams indicate that permeability was the major mechanism of resistance to the poorly hydrolyzed and nonhydrolyzed antibiotics, e.g., carbenicillin, moxalactam, and cefsulodin. An exception was cefotaxime, with a low Km and a low Vmax, which had reduced efficacy in the permeability mutant and was further affected by the constitutive beta-lactamase. In this case, since the Vmax was low, a nonhydrolytic barrier may provide the additional reduction in susceptibility.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference26 articles.
1. Role of a cefoxitininducible beta-lactamase in a case of breakthrough bacteremia;Beckwith D. G.;J. Clin. Microbiol.,1980
2. Resistance of Pseudomonas aeruginosa mutants with altered control of chromosomal P-lactamase to piperacillin, ceftazidime, and cefsulodin;Bryan L. E.;Antimicrob. Agents Chemother.,1984
3. Bush K. and R. B. Sykes. 1982. Interaction of new beta-lactams with beta-lactamases and beta-lactamase-producing gram-negative rods p. 47-63. In H. C. Neu (ed.) New beta-lactam antibiotics: a review from chemistry to clinical efficacy of the new cephalosporins. Frances Clark Wood Institute for the History of Medicine Philadelphia.
4. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains;Darveau R. P.;J. Bacteriol.,1983
5. Gillett A. P. 1982. Antibiotics against Pseudomonas. J. Antimicrob. Chemother. 9(Suppl. B):41-48.
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